Figure 1a: Inverted experimental plate with E. coli BL21(DE3), plasmid pGEM-gbr22 C grown on agar plate with ampicillin after placed in 37˚C incubator for 24 hours. Light purple dots on the agar represent each growing E. coli bacterial colony. Water droplets on plate resulted from condensation. Plate is labeled with sharpie as experimental plate with VDS, initials, date, BL21(DE3), Amp, and plasmid pGEM-gbr22 C.
Figure 1b: Inverted control plate with E. coli BL21(DE3) and NO plasmid grown on agar plate with ampicillin after placed in 37˚C incubator for 24 hours. Light purple dots on the agar represent each growing E. coli bacterial colony. Water droplets on plate resulted from condensation. Plate is labeled with sharpie as control plate with VDS, initials, date, BL21(DE3), Amp, and no presence of plasmid.
Figure 1c: Inverted “fun” plate with swabbed mucus from student grown on agar plate with LB and no ampicillin after placed in 37˚C incubator for 24 hours. Water droplets on plate resulted from condensation. Plate is labeled with sharpie as fun plate with VDS, initials, date, and LB +agar.
Figure 1d: Inverted experimental plate with E. coli BL21(DE3), plasmid pGEM-gbr22 C grown on agar plate with ampicillin after placed in 37˚C incubator for 32 hours. Light purple dots on the agar represent each growing E. coli bacterial colony. This image was taken after plate was placed back in the incubator for 8 more hours to allow for more growth. Plate is labeled with sharpie as experimental plate with VDS, initials, date, BL21(DE3), Amp, and plasmid pGEM-gbr22 C.
Figure 2: Transformed purple culture of E. coli BL21(DE3) with plasmid C pGEM-gbr22 in an aluminum-foil-covered 125 mL Erlenmeyer flask containing LB and ampicillin after placed in a shaker incubator for 16-24 hours at in 37˚C. Flask labeled with initials, VDS, date, LB+Amp, BL21(DE3) and plasmid pGEM-gbr22 type C.
Figure 3: Post-centrifugation purple wet pellet excluding supernatant fluid of transformed purple culture of E. coli BL21(DE3) with plasmid C pGEM-gbr22. The culture was centrifuged for 10 minutes in a in 4˚C benchtop centrifuge at 5000 rpm speed. Conical tube was labeled with initials, VDS, date, LB+Amp, BL21(DE3) and plasmid pGEM-gbr22 type C. Measured pellet weight is 0.93 g.
Introduction:
Materials & Methods:
Results:
Discussion:
Conclusions:
References: